Discusses the metabolites of sulfur mustard can be readily and safely synthesised and used as reference standards to assists in identification and verification of sulfur mustard in various sample matrices, contaminated with sulfur mustard. The objectives of the study were: 1. To develop a procedure for the synthesis, purification and characterisation of the bis(2-hydroxyethyl) sulfoxide and its sulfone analogue. 2. To synthesise sulfur mustard metabolites from the beta-lyase pathway i.e. 1-methylsulfinyl-2-[2-(methylthioethyl)sulfonyl] ethane (MSMTESE) and 1,1-sulfonylbis[(methylsulfinyl) ethane] (SBMSE) starting from the thiodiglycol. 3. To synthesise 1,1-sulfonylbis[(2-S-(N-cysteinyl) ethane] (SBSNAE) from bis(2-chloroethyl) sulfone and glutathione, and to oxidise it to 1,1-sulfonyl bis[2-S-(N-acetylcysteinyl) ethane]. 4. To develop chromatographic methods of analysis for the synthesised by-products.