Scale samples from Temminck’s ground pangolin (Smutsia temminckii): a non-invasive source of DNA.

Home
Show All
Show Quick Collection
Highlights +
Most Accessed Papers Most Accessed Items Most Accessed Authors Recent Additions
Search History
Clear Session
Library
Help

Change Site View +
GlobalView Institutes and Directorates

Du Toit, Zelda.

Home

Faculty of Science

Department of Environmental Water and Earth Science - Research Articles

Add to Quick Collection

Du Toit, Zelda., Grobler, J. Paul., Kotze, Antoinette., Jansen, Raymond.. Conservation Genetics Resources; August 216. Scale samples from Temminck’s ground pangolin (Smutsia temminckii): a non-invasive source of DNA..

File	Description	Size	Format
SOURCE1	Du Toit, Z..pdf	794 KB	Adobe Acrobat PDF	View Details	Download

Title: Scale samples from Temminck’s ground pangolin (Smutsia temminckii): a non-invasive source of DNA.
Creator: Du Toit, Zelda.
Creator: Grobler, J. Paul.
Creator: Kotze, Antoinette.
Creator: Jansen, Raymond.
Subject: DNA isolation.
Subject: Non-invasive sampling.
Subject: PCR optimization.
Subject: Scale samples.
Subject: Temminck’s ground pangolin.
Date: August 216
Publisher: Conservation Genetics Resources
Description: Non-invasive sampling aims to obtain suitable samples without causing harm to the study organism, thus making it a more preferred sampling technique. Molecular methods have advanced in such a way that quality DNA can be obtained from less invasive mammal samples. Various PCR enhancing techniques have also been developed to aid in amplification of low quality DNA samples, to produce reliable results that can be used to monitor populations and species or for forensic applications. Temminck’s ground pangolin is one of the few mammals to be covered in scales. In this study, we attempt to identify the most appropriate DNA isolation and PCR methods as well as determine whether PCR additives could be used to improve isolation of DNA and downstream amplification of mitochondrial DNA, from scale samples collected from these pangolins. In this study, it was determined that several different methods are required in order to successfully amplify DNA, and a decision tree rather than a single method should be followed.

Relationships: Show Relationship Browser for this Object
collection(s): Department of Environmental Water and Earth Science - Research Articles
Identifier: tut:5251
Format: PDF
Language: en
RightsHolder: Springer Science+Business Media
Type: Text
Full Text

573 Visitors

605 Hits

2 Downloads

Department of Environmental Water and Earth Science - Research Articles Scale samples from Temminck’s ground pangolin (Smutsia temminckii): a non-invasive source of DNA.

Vital Repository 7.0