Magnetic activated cell sorting versus density gradient centrifugation in assisted reproductive technology.
Jardim, Jenna Queiroz Nunes
Jardim, Jenna Queiroz Nunes
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Abstract
Background:
Sperm deoxyribonucleic acid fragmentation has been linked to decreased fertility. Magnetic activated cell sorting selects intact spermatozoa with optimal characteristics, utilizing specific membrane surface markers to remove apoptotic spermatozoa, reducing the proportion with deoxyribonucleic acid fragmentation.
Objectives:
To investigate the effect of magnetic activated cell sorting after density gradient centrifugation on sperm motility, morphology, and deoxyribonucleic acid
fragmentation.
Methods:
A randomised controlled study including 45 male patients. Density gradient centrifugation (control) versus density gradient centrifugation with magnetic activated cell sorting (experiment) were compared using computer aided sperm analysis for sperm kinematics, RapiDiff staining for morphology, and a sperm
chromatin dispersion test for sperm deoxyribonucleic acid fragmentation.
Results:
The experimental group showed significantly less sperm deoxyribonucleic acid fragmentation (p < 0.001) than the control. There were significant differences
in Teratozoospermia Index, total sperm concentration and progressive motile concentration (all p < 0. 001), with comparable results for sperm motility, progressive motility, and normal forms (all p > 0.05).
Conclusion:
Despite good motility and normal morphologic appearance, a spermatozoon may have fragmented deoxyribonucleic acid, and if used during in vitro fertilization could result in poor outcomes. Magnetic activated cell sorting reduced both sperm deoxyribonucleic acid fragmentation and the average number of defects per abnormal spermatozoon without impairing sperm motility.
Description
Submitted in partial fulfilment of the requirements for the degree, Magister Technologiae: Clinical Technology Reproductive Biology in the Department of Biomedical Sciences, Faculty of Science at the Tshwane University of Technology.
Date
2020-10-01
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Tshwane University of Technology
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Keywords
Sperm deoxyribonucleic acid fragmentation, Density gradient centrifugation, Magnetic activated cell sorting and apoptotic spermatozoa