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Isolation of rhizobium meliloti nod gene inducers from alfalfa rhizosphere soil.
Leon-Barrios, Milagros ; Dakora, Felix D. ; Josoeph, Cecillia M. ; Phillips, Donald A.
Leon-Barrios, Milagros
Dakora, Felix D.
Josoeph, Cecillia M.
Phillips, Donald A.
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Abstract
Methanolic extracts of alfalfa rhizosphere soil induce more nod gene transcription in Rhizobium meliloti than extracts of nonrhizosphere soil. Six peaks of nod-inducing activity were separated by high-performance liquid chromatography from rhizosphere soil extract, and one compound was identified by 'H nuclear magnetic resonance, mass spectrometry, and UV-visible spectra as a formononetin-7-O-glycoside that activates both NodDl and NodD2 proteins. The unanticipated presence of a glycoside in rhizosphere soil suggests either that large amounts of the glycoside were exuded by roots or that some glycosides are unexpectedly stable in soil.
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1992-11-24
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American Society for Microbiology
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Dakora 66.pdf
Adobe PDF, 717.9 KB
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Alfalfa rhizosphere, Rhizobium meliloti, NodDl and NodD2 proteins
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Attribution-NonCommercial-ShareAlike 4.0 International
