Comparison of the preservation protocols for windsnyer boars semen.
Thema, Mamonene Angelinah
Thema, Mamonene Angelinah
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Abstract
Boar semen preservation is associated with a reduction in sperm viability and fertilizing capacity caused by cold shock, oxidative stress, and crystal formation induced during preservation. Semen was collected from three Windsnyer boars of known fertility. Collected semen samples were evaluated for macroscopic and microscopic traits then pooled and allocated randomly per treatment group. Semen samples were diluted with semen extenders and equilibrated at two in vitro storage temperatures. Cooled semen samples were centrifuged, and semen pellets were diluted with cryoprotectant concentrations and then loaded into straws. Two cryopreservation methods were used to cryopreserve the semen straws. Semen straws were thawed at different temperatures and evaluated for sperm motility, viability, and morphology traits. The Beltsville Thawing Solution extender treatments maintained the highest sperm total motility percentage at 8 (83.4±2.4), 12 (78.0±3.8), 24 (58.0±12.6), and 48 (13.0±14.1) hours when stored at 18ºC (P<0.05). Post-thawed sperm total motility (36.0±5.3) and live normal sperm (49.5±8.3) percentages were recorded to be higher in the treatment supplemented with 16% Glycerol (P<0.05). The highest sperm total motility percentage was recorded at 40ºC (26.8±3.2) thawing temperature for liquid nitrogen vapour treatment (P<0.05). In conclusion, the Beltsville Thawing Solution extender maintained the sperm motility, viability, and morphology percentages over 48 hours when was preserved at 18ºC. Additionally, 16% Glycerol was found to be the suitable cryoprotectant concentration for the semen cryopreserved with the liquid nitrogen vapour as a cryopreservation method and thawed at 40ºC.
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Submitted in partial fulfilment of the requirement for the degree, Master of Agricultural Science in the Department of Animal Sciences, Faculty of Science at the Tshwane University of Technology
Date
2022
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Tshwane University of Technology
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Keywords
Boar semen preservation, Fertility, Cryopreservation methods, Sperm viability, Protocols, Windsnyer boars