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Study the efficacy of different concentrations of coconut water on boar semen following equilibration at 18°C for different hours.

Tshabalala, Mduduzi M.
Mphaphathi, Masindi L.
Netshirovha, Thivhilaheli R.
Nephawe, Khathutshedzo A.
Pilane, Cyril M.
Nedambale, Lucky
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Abstract
A rich natural resource such as Coconut Water (CCW) features high concentrations of antioxidants, nutrients, amino acids, sugars, and electrolytes. These herbal extract infusions are used in semen extenders to preserve animal sperm. In addition, CCW is important for in vitro production of embryos. The purpose of this study was to investigate the use of coconut water as an alternative bulking agent for boar semen after equilibration at 18° for 8, 16, 24, and 48 h. Three 2- to 3-year-old Large White x Landrace boars were used for semen collection, boar semen was collected by a glove and hand technique, 24 ejaculates were collected and this study was replicated eight times. A complete randomized design was used in this study. Data were analyzed by ANOVA using Stata V12 statistical software (Stata Corp., College Station, Tex.) and treatment measures were separated using Fisher's protected t-test at significance values of P<0.05. Sperm diluted in 60% CCW and equilibrated for 8 h showed improved sperm motility (86.1±1.7), percentage of viable sperm (84.5±20), plasma membrane (83.3±2.3), and acrosome integrity (84.00±2.13) compared to boar semen diluted with 80 and 100% CCW. Notably, semen diluted in 80 and 100% CCW and equilibrated for 8 h showed improved sperm motility (76.2±2.5; 63.5±2.7), viable sperm (74.5±2.0; 63.3±2.1), intact plasma membrane (73.3±23; 63.5±2.0) and acrosomal membrane integrity after equilibration (74.0±2.1; 63.5±2.7). However, sperm diluted in 80 and 100% CCW had reduced sperm motility, viability, and plasma and acrosomal membrane integrity after 8, 16, 24 and 48 h of equilibration at 18°C. In conclusion, equilibrated boar semen diluted with 60% CCW was able to maintain boar semen parameters such as sperm motility, viability, plasma membrane, and acrosomal membrane integrity following 8, 16, 24, and 48 of equilibration at 18°C. However, CCW can be used as an alternative extender for the equilibration of boar semen. Therefore, further studies are necessary to determine the in vitro and in vivo fertilizing capacity of equilibrium boar semen diluted in CCW.
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Date
2022-03-02
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Science Publications
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Keywords
Acrosome Integrity, Boar Semen, Coconut Water, Equilibration, Sperm Viability Plasma Membrane Integrity
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