Adebayo, Salmon A.Shai, Leshweni J.Eloff, Jacobus N.2024-10-312024-10-312016-12-151995-7645http://dx.doi.org/10.1016/j.apjtm.2016.12.004https://hdl.handle.net/20.500.14519/969Objective: To investigate the anti-inflammatory activity of different fractions and glutinol (isolated compound), using nitric oxide synthase and cyclooxygenase (COX) inhibition as an indication of anti-inflammatory activity. Methods: Anti-inflammatory activity was evaluated using an in vitro assay determining the inhibition of the activity of pro-inflammatory enzyme model. Cyclooxygenases and inducible nitric oxide synthase are crucial enzymes involved in the pathogenesis of many chronic inflammatory conditions. Results: Sub-fraction F3.3 that was derived from n-hexane fraction of PA leaves significantly inhibited (P = 0.01) the catalytic activity of COX-2 (IC50 = 0.67 mg/mL) better than isolated compound, glutinol (IC50 = 1.22 mg/mL), compound 2 (CP2) (IC50 = 1.71 mg/mL) and sub-fraction F3.3.0 (IC50 = 1.30 mg/mL). A similar trend was observed in investigation of the inhibition of nitric oxide synthesis in RAW 264.7 cells by glutinol, CP2 and F3.3.0. Inducible COX-2 and inducible nitric oxide synthase are among potent signalling enzymes that exacerbate inflammation. Conclusions: Bioactive sub-fractions (F3.3 and F3.3.0) derived from the n-hexane fraction of PA had good anti-inflammatory activity, and the isolated compound, and glutinol may be useful as a template for the development of new anti-inflammatory drugs.42-46 PagesenAttribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/Peltophorum africanumCOX-1/COX-2iNOSNitric oxideAnti-inflammatoryArticle