Investigation of the mechanism of anti- inflammatory action and cytotoxicity of a semipurified fraction and isolated compounds from the leaf of peltophorum africanum (Fabaceae).
Adebayo, Salmon A. Steel, Helen C. Shai, Leshweni J. Eloff, Jacobus N.
Adebayo, Salmon A.
Steel, Helen C.
Shai, Leshweni J.
Eloff, Jacobus N.
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Abstract
Peltophorum africanum extracts have been shown to possess many important medicinal benefits, including anti-inflammatory and antiviral activities. However, the mechanism of action is poorly understood. The mechanism of anti-inflammatory action was determined by measuring the synthesis of cytokines in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophage cells in vitro. Compound 1 (CP1), compound 2 (CP2), and fraction F3.3.0 (F3.3.0) significantly reduced the synthesis of interleukin 1b (IL-1b) from RAW 264.7 cells (1.18, 1.32, and 0.92 ng/mL), respectively. Similarly, CP1, CP2, and F3.3.0 inhibited the production of IL-2 and tumor necrosis factor–a (TNF-a) by RAW 264.7 cells (0.41, 0.60, 0.74 and 0.11, 0.27, 0.24 ng/mL, respectively. In addition, CP1 and CP2 had lower cytotoxicity toward RAW 264.7 cells, with CP2 indicating the lowest cytotoxicity (LD50 ¼ 207.88 mg/mL). The mechanism of action was found to be via the inhibition of pro-inflammation cytokines (IL-1 b and TNF-a). This observation may support the use of P africanum to treat pain-related conditions
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Date
2017-05-19
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SAGE
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Keywords
IL-1b, IL-2, TNF-a, Anti-inflammatory, RAW 264.7, Peltophorum africanum